6S2E

Cryo-EM structure of Ctf18-1-8 in complex with the catalytic domain of DNA polymerase epsilon

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase epsilon catalytic subunit A	polymer	1213	139618.4	1	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) In PDB	Saccharomyces cerevisiae S288C	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
2	D	Chromosome transmission fidelity protein 8	polymer	133	15189.7	1	UniProt (P38877) Pfam (PF09696) In PDB	Saccharomyces cerevisiae S288C
3	E	Chromosome transmission fidelity protein 18	polymer	33	3859.3	1	UniProt (P49956) In PDB	Saccharomyces cerevisiae S288C
4	B	Sister chromatid cohesion protein DCC1	polymer	380	44133.8	1	UniProt (P25559) Pfam (PF09724) In PDB	Saccharomyces cerevisiae S288C	Defective in sister chromatid cohesion protein 1
5	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 1 - 1186 (UniProt: P21951)

PDB	External Database	Details
Met -26	-	initiating methionine
Lys -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Ser -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Leu -15	-	expression tag
Glu -14	-	expression tag
Val -13	-	expression tag
Leu -12	-	expression tag
Phe -11	-	expression tag
Gln -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Thr -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Glu -1	-	expression tag
Leu 0	-	expression tag
Ala 290	Asp 290	engineered mutation
Ala 292	Glu 292	engineered mutation

E: 713 - 741 (UniProt: P49956)

PDB	External Database	Details
Gly 709	-	expression tag
Ala 710	-	expression tag
Met 711	-	expression tag
Gly 712	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	202801.2
Non-Polymers*	Number of molecules	1
	Total formula weight	351.6
All*	Total formula weight	203152.8

*Water molecules are not included.