6S2E
Cryo-EM structure of Ctf18-1-8 in complex with the catalytic domain of DNA polymerase epsilon
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase epsilon catalytic subunit A | polymer | 1213 | 139618.4 | 1 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) In PDB | Saccharomyces cerevisiae S288C | 3'-5' exodeoxyribonuclease,DNA polymerase II subunit A |
2 | D | Chromosome transmission fidelity protein 8 | polymer | 133 | 15189.7 | 1 | UniProt (P38877) Pfam (PF09696) In PDB | Saccharomyces cerevisiae S288C | |
3 | E | Chromosome transmission fidelity protein 18 | polymer | 33 | 3859.3 | 1 | UniProt (P49956) In PDB | Saccharomyces cerevisiae S288C | |
4 | B | Sister chromatid cohesion protein DCC1 | polymer | 380 | 44133.8 | 1 | UniProt (P25559) Pfam (PF09724) In PDB | Saccharomyces cerevisiae S288C | Defective in sister chromatid cohesion protein 1 |
5 | A | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) |
Sequence modifications
A: 1 - 1186 (UniProt: P21951)
E: 713 - 741 (UniProt: P49956)
PDB | External Database | Details |
---|---|---|
Met -26 | - | initiating methionine |
Lys -25 | - | expression tag |
His -24 | - | expression tag |
His -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
Ser -18 | - | expression tag |
Ala -17 | - | expression tag |
Gly -16 | - | expression tag |
Leu -15 | - | expression tag |
Glu -14 | - | expression tag |
Val -13 | - | expression tag |
Leu -12 | - | expression tag |
Phe -11 | - | expression tag |
Gln -10 | - | expression tag |
Gly -9 | - | expression tag |
Pro -8 | - | expression tag |
Gly -7 | - | expression tag |
Thr -6 | - | expression tag |
Gly -5 | - | expression tag |
Ser -4 | - | expression tag |
Glu -3 | - | expression tag |
Phe -2 | - | expression tag |
Glu -1 | - | expression tag |
Leu 0 | - | expression tag |
Ala 290 | Asp 290 | engineered mutation |
Ala 292 | Glu 292 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 709 | - | expression tag |
Ala 710 | - | expression tag |
Met 711 | - | expression tag |
Gly 712 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 202801.2 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 351.6 | |
All* | Total formula weight | 203152.8 |