6S2E
Cryo-EM structure of Ctf18-1-8 in complex with the catalytic domain of DNA polymerase epsilon
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase epsilon catalytic subunit A | polymer | 1213 | 139618.4 | 1 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) | Saccharomyces cerevisiae S288C | 3'-5' exodeoxyribonuclease,DNA polymerase II subunit A |
| 2 | B (D) | Chromosome transmission fidelity protein 8 | polymer | 133 | 15189.7 | 1 | UniProt (P38877) Pfam (PF09696) | Saccharomyces cerevisiae S288C | |
| 3 | C (E) | Chromosome transmission fidelity protein 18 | polymer | 33 | 3859.3 | 1 | UniProt (P49956) | Saccharomyces cerevisiae S288C | |
| 4 | D (B) | Sister chromatid cohesion protein DCC1 | polymer | 380 | 44133.8 | 1 | UniProt (P25559) Pfam (PF09724) | Saccharomyces cerevisiae S288C | Defective in sister chromatid cohesion protein 1 |
| 5 | E (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) |
Sequence modifications
A: 1 - 1186 (UniProt: P21951)
E: 713 - 741 (UniProt: P49956)
| PDB | External Database | Details |
|---|---|---|
| Met -26 | - | initiating methionine |
| Lys -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Leu -15 | - | expression tag |
| Glu -14 | - | expression tag |
| Val -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Phe -11 | - | expression tag |
| Gln -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Thr -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Ala 290 | Asp 290 | engineered mutation |
| Ala 292 | Glu 292 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 709 | - | expression tag |
| Ala 710 | - | expression tag |
| Met 711 | - | expression tag |
| Gly 712 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 202801.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 351.6 | |
| All* | Total formula weight | 203152.8 |
