6RTW

Crystal structure of the Patched-1 (PTCH1) ectodomain in complex with nanobody NB64 and cholesterol-hemisuccinate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein patched homolog 1	polymer	297	34138.8	1	UniProt (Q13635) In PDB	Homo sapiens (Human)	PTC1
2	B	Llama-derived nanobody NB64	polymer	130	14043.6	1		Lama glama
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	A	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
5		water	water		18.0	210	Chemie (HOH)

Sequence modifications

A: 136 - 423 (UniProt: Q13635)

PDB	External Database	Details
Glu 136	Val 136	conflict
Thr 137	Ser 137	conflict
Gly 138	Arg 138	conflict
Gly 424	-	expression tag
Thr 425	-	expression tag
Lys 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48182.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1150.3
All*	Total formula weight	49332.7

*Water molecules are not included.