6RQC

Cryo-EM structure of an MCM loading intermediate

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Origin recognition complex subunit 1	polymer	949	108612.9	1	UniProt (P54784) Pfam (PF01426) Pfam (PF00004) Pfam (PF17872) Pfam (PF21312)	Saccharomyces cerevisiae S288c (Baker's yeast)	Origin recognition complex 120 kDa subunit
2	B (B)	Origin recognition complex subunit 2	polymer	620	71342.2	1	UniProt (P32833) Pfam (PF04084)	Saccharomyces cerevisiae S288c (Baker's yeast)	Origin recognition complex 71 kDa subunit
3	C (C)	Origin recognition complex subunit 3	polymer	616	72161.8	1	UniProt (P54790) Pfam (PF07034) Pfam (PF18137)	Saccharomyces cerevisiae S288c (Baker's yeast)	Origin recognition complex 62 kDa subunit
4	D (D)	Origin recognition complex subunit 4	polymer	529	60772.2	1	UniProt (P54791) Pfam (PF14629)	Saccharomyces cerevisiae S288c (Baker's yeast)	Origin recognition complex 56 kDa subunit
5	E (E)	Origin recognition complex subunit 5	polymer	479	55347.2	1	UniProt (P50874) Pfam (PF13191) Pfam (PF21639) Pfam (PF14630)	Saccharomyces cerevisiae S288c (Baker's yeast)	Origin recognition complex 53 kDa subunit
6	F (F)	Origin recognition complex subunit 6	polymer	435	50369.5	1	UniProt (P38826) Pfam (PF05460)	Saccharomyces cerevisiae S288c (Baker's yeast)	ACS-associated protein 1,Origin recognition complex 50 kDa subunit
7	G (2)	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae S288c (Baker's yeast)	Minichromosome maintenance protein 2
8	H (3)	DNA replication licensing factor MCM3	polymer	1006	111720.2	1	UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae S288c (Baker's yeast)	Minichromosome maintenance protein 3
9	I (4)	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Saccharomyces cerevisiae S288c (Baker's yeast)	Cell division control protein 54
10	J (5)	Minichromosome maintenance protein 5	polymer	775	86505.7	1	UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933)	Saccharomyces cerevisiae S288c (Baker's yeast)	Cell division control protein 46
11	K (6)	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae S288c (Baker's yeast)	Minichromosome maintenance protein 6
12	L (7)	DNA replication licensing factor MCM7	polymer	845	95049.9	1	UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae S288c (Baker's yeast)	Cell division control protein 47,Minichromosome maintenance protein 7
13	M (X)	DNA (88-MER)	polymer	88	27116.3	1		Saccharomyces cerevisiae S288c
14	N (Y)	DNA (88-MER)	polymer	88	27154.6	1		Saccharomyces cerevisiae S288c
15	O, Q, S (A, D, E)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
16	P, R, T (A, D, E)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
17	BA, U, W, Y (7, 2, 3, 5)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
18	AA, CA, V, X, Z (6, 7, 2, 4, 5)	ZINC ION	non-polymer		65.4	5	Chemie (ZN)

Sequence modifications

A: 1 - 914 (UniProt: P54784)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

3: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	1083312.6
Non-Polymers*	Number of molecules	15
	Total formula weight	3630.3
All*	Total formula weight	1086942.9

*Water molecules are not included.