6RNK

Crystal structure of a humanized (K18E, K269N) rat succinate receptor SUCNR1 (GPR91) in complex with a nanobody and antagonist NF-56-EJ40.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Succinate receptor 1	polymer	342	39827.7	1	UniProt (Q6IYF9) Pfam (PF00001)	Rattus norvegicus (Rat)	G-protein coupled receptor 91
2	B (B)	Nanobody6	polymer	142	15527.1	1		Vicugna pacos
3	C (A)	2-[2-[[3-[4-[(4-methylpiperazin-1-yl)methyl]phenyl]phenyl]carbonylamino]phenyl]ethanoic acid	non-polymer		443.5	1	Chemie (KAZ)
4	D, E, F, G, H... (A)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	13	Chemie (OLC)
5	Q (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	R (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	S, T (A, B)	water	water		18.0	242	Chemie (HOH)

Sequence modifications

A: 2 - 317 (UniProt: Q6IYF9)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag
Glu 18	Lys 18	engineered mutation
Asn 269	Lys 269	engineered mutation
Leu 318	-	expression tag
Glu 319	-	expression tag
Val 320	-	expression tag
Leu 321	-	expression tag
Phe 322	-	expression tag
Gln 323	-	expression tag
Gly 324	-	expression tag
Pro 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55354.8
Non-Polymers*	Number of molecules	16
	Total formula weight	5266.7
All*	Total formula weight	60621.5

*Water molecules are not included.