6RFP

ERK2 MAP kinase with mutations at Helix-G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mitogen-activated protein kinase 1	polymer	378	43425.7	1	UniProt (P63086) Pfam (PF00069) In PDB	Rattus norvegicus (Rat)	MAPK 1,ERT1,Extracellular signal-regulated kinase 2,ERK-2,MAP kinase isoform p42,p42-MAPK,Mitogen-activated protein kinase 2,MAPK 2
2		water	water		18.0	227	Chemie (HOH)

Sequence modifications

A: 2 - 358 (UniProt: P63086)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 1	-	expression tag
Thr 229	Lys 229	engineered mutation
Asp 230	His 230	engineered mutation
His 231	Tyr 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43425.7
All*	Total formula weight	43425.7

*Water molecules are not included.