6RB7

Ruminococcus gnavus sialic acid aldolase catalytic lysine mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, E, F)	Putative N-acetylneuraminate lyase	polymer	336	37103.6	4	UniProt (A7B555)	[Ruminococcus] gnavus ATCC 29149
2	E, L, O, R (A, B, E, F)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	4	Chemie (TRS)
3	F, H, J, M, P... (A, B, E, F)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
4	G, T, U (A, F)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
5	I (A)	BICINE	non-polymer		163.2	1	Chemie (BCN)
6	K, N, Q, V (A, B, E, F)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	W, X, Y, Z (A, B, E, F)	water	water		18.0	1826	Chemie (HOH)

Sequence modifications

A, B, E, F: 1 - 305 (UniProt: A7B555)

PDB	External Database	Details
Met -30	-	initiating methionine
Ser -29	-	expression tag
Tyr -28	-	expression tag
Tyr -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Asp -18	-	expression tag
Ile -17	-	expression tag
Pro -16	-	expression tag
Thr -15	-	expression tag
Thr -14	-	expression tag
Glu -13	-	expression tag
Asn -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Ala -6	-	expression tag
Met -5	-	expression tag
Ala -4	-	expression tag
Asp -3	-	expression tag
Ile -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 167	Lys 177	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148414.2
Non-Polymers*	Number of molecules	18
	Total formula weight	1619.9
All*	Total formula weight	150034.1

*Water molecules are not included.