6R90

Cryo-EM structure of NCP-THF2(+1)-UV-DDB class A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.1	polymer	139	15719.4	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F (B, F)	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I (I)	Human alpha-satellite DNA (145-MER)	polymer	145	44756.6	1		Homo sapiens
6	J (J)	Human alpha-satellite DNA (145-MER) with abasic sites at positions 93-94	polymer	145	44452.4	1		Homo sapiens
7	K (K)	DNA damage-binding protein 1	polymer	1163	129766.3	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	Homo sapiens (Human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
8	L (L)	DNA damage-binding protein 2	polymer	450	50601.8	1	UniProt (Q92466) Pfam (PF00400)	Homo sapiens (Human)	DDB p48 subunit,DDBb,Damage-specific DNA-binding protein 2,UV-damaged DNA-binding protein 2,UV-DDB 2

Sequence modifications

A, E: 1 - 136 (UniProt: P68431)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B, F: 1 - 103 (UniProt: P62805)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

C, G: 1 - 130 (UniProt: P04908)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

D, H: 1 - 126 (UniProt: P06899)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

K: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Met -22	-	initiating methionine
Ala -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

L: 1 - 427 (UniProt: Q92466)

PDB	External Database	Details
Met -22	-	initiating methionine
Ala -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	381700.2
All*	Total formula weight	381700.2

*Water molecules are not included.