6R90
Cryo-EM structure of NCP-THF2(+1)-UV-DDB class A
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.5 |
| Software | RELION,REFMAC,EPU,PHENIX,Gctf,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
