6R59
Crystal structure of PPEP-1(E143A/Y178F) in complex with substrate peptide Ac-EVAPPVP-NH2
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Pro-Pro endopeptidase | polymer | 197 | 21822.6 | 2 | UniProt (Q183R7) Pfam (PF07737) In PDB | Peptoclostridium difficile | PPEP-1,Zinc metalloprotease Zmp1 |
2 | E, C | ACE-GLU-VAL-ALA-PRO-PRO-VAL-LPD | polymer | 8 | 732.9 | 2 | Clostridioides difficile | ||
3 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
4 | water | water | 18.0 | 268 | Chemie (HOH) |
Sequence modifications
A, B: 27 - 220 (UniProt: Q183R7)
PDB | External Database | Details |
---|---|---|
Gly 24 | - | expression tag |
His 25 | - | expression tag |
Met 26 | - | expression tag |
Ala 143 | Glu 143 | engineered mutation |
Phe 178 | Tyr 178 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 45110.9 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 130.8 | |
All* | Total formula weight | 45241.7 |