6R58
Crystal structure of PPEP-1(E143A/Y178F/E184A) in complex with substrate peptide Ac-EVNAPVP-CONH2
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Pro-Pro endopeptidase | polymer | 198 | 21851.6 | 4 | UniProt (Q183R7) Pfam (PF07737) In PDB | Peptoclostridium difficile | PPEP-1,Zinc metalloprotease Zmp1 |
2 | E, F, I, G | ACE-GLU-VAL-ASN-ALA-PRO-VAL-LPD | polymer | 8 | 749.9 | 4 | Clostridioides difficile | ||
3 | A, C, D | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
4 | B | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
5 | D | NICKEL (II) ION | non-polymer | 58.7 | 1 | Chemie (NI) | |||
6 | water | water | 18.0 | 448 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 27 - 220 (UniProt: Q183R7)
PDB | External Database | Details |
---|---|---|
Gly 23 | - | expression tag |
Ser 24 | - | expression tag |
His 25 | - | expression tag |
Met 26 | - | expression tag |
Ala 143 | Glu 143 | engineered mutation |
Phe 178 | Tyr 178 | engineered mutation |
Ala 184 | Glu 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 90405.9 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 349.9 | |
All* | Total formula weight | 90755.8 |