6R58
Crystal structure of PPEP-1(E143A/Y178F/E184A) in complex with substrate peptide Ac-EVNAPVP-CONH2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Pro-Pro endopeptidase | polymer | 198 | 21851.6 | 4 | UniProt (Q183R7) Pfam (PF07737) | Peptoclostridium difficile | PPEP-1,Zinc metalloprotease Zmp1 |
| 2 | E, F, G, H (E, F, I, G) | ACE-GLU-VAL-ASN-ALA-PRO-VAL-LPD | polymer | 8 | 749.9 | 4 | Clostridioides difficile | ||
| 3 | I, K, L (A, C, D) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 4 | J (B) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 5 | M (D) | NICKEL (II) ION | non-polymer | 58.7 | 1 | Chemie (NI) | |||
| 6 | N, O, P, Q, R... (A, B, C, D, E...) | water | water | 18.0 | 448 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 27 - 220 (UniProt: Q183R7)
| PDB | External Database | Details |
|---|---|---|
| Gly 23 | - | expression tag |
| Ser 24 | - | expression tag |
| His 25 | - | expression tag |
| Met 26 | - | expression tag |
| Ala 143 | Glu 143 | engineered mutation |
| Phe 178 | Tyr 178 | engineered mutation |
| Ala 184 | Glu 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 90405.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 349.9 | |
| All* | Total formula weight | 90755.8 |
