6R58

Crystal structure of PPEP-1(E143A/Y178F/E184A) in complex with substrate peptide Ac-EVNAPVP-CONH2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008237	molecular_function	metallopeptidase activity
A	0046872	molecular_function	metal ion binding
B	0005576	cellular_component	extracellular region
B	0006508	biological_process	proteolysis
B	0008237	molecular_function	metallopeptidase activity
B	0046872	molecular_function	metal ion binding
C	0005576	cellular_component	extracellular region
C	0006508	biological_process	proteolysis
C	0008237	molecular_function	metallopeptidase activity
C	0046872	molecular_function	metal ion binding
D	0005576	cellular_component	extracellular region
D	0006508	biological_process	proteolysis
D	0008237	molecular_function	metallopeptidase activity
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 300

Chain	Residue
A	HIS142
A	HIS146
A	GLU185
E	ALA4

---

site_id	AC2
Number of Residues	4
Details	binding site for residue PO4 B 301

Chain	Residue
B	SER119
B	HIS150
F	ACE0
F	GLU1

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN C 300

Chain	Residue
C	HIS146
C	GLU185
I	ALA4
C	HIS142

---

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN D 301

Chain	Residue
D	HIS142
D	HIS146
D	GLU185
G	ALA4

---

site_id	AC5
Number of Residues	4
Details	binding site for residue NI D 302

Chain	Residue
D	GLY23
D	SER24
D	HIS25
D	MET26

---

site_id	AC6
Number of Residues	6
Details	binding site for Di-peptide ACE E 0 and GLU E 1

Chain	Residue
A	SER119
A	HIS150
A	HOH456
E	VAL2
E	ASN3
E	HOH105

---

site_id	AC7
Number of Residues	7
Details	binding site for Di-peptide VAL E 6 and LPD E 7

Chain	Residue
A	HIS134
A	ASP135
A	HIS142
A	PHE178
B	GLY105
E	PRO5
E	HOH101

---

site_id	AC8
Number of Residues	7
Details	binding site for Di-peptide ACE F 0 and GLU F 1

Chain	Residue
B	GLY118
B	SER119
B	HIS150
B	PO4301
F	VAL2
F	ASN3
F	HOH102

---

site_id	AC9
Number of Residues	6
Details	binding site for Di-peptide VAL F 6 and LPD F 7

Chain	Residue
B	GLY102
B	HIS134
B	ASP135
B	ASN175
B	PHE178
F	PRO5

---

site_id	AD1
Number of Residues	6
Details	binding site for Di-peptide ACE G 0 and GLU G 1

Chain	Residue
B	TYR94
D	GLY118
D	SER119
D	HIS150
G	VAL2
G	ASN3

---

site_id	AD2
Number of Residues	7
Details	binding site for Di-peptide VAL G 6 and LPD G 7

Chain	Residue
C	GLY105
D	GLY102
D	HIS134
D	ASP135
D	HIS142
D	PHE178
G	PRO5

---

site_id	AD3
Number of Residues	6
Details	binding site for Di-peptide ACE I 0 and GLU I 1

Chain	Residue
A	THR30
C	GLY118
C	SER119
C	HOH429
I	VAL2
I	ASN3

---

site_id	AD4
Number of Residues	7
Details	binding site for Di-peptide VAL I 6 and LPD I 7

Chain	Residue
C	GLY102
C	TRP103
C	HIS134
C	ASP135
C	ALA136
C	PHE178
I	PRO5

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU01339, ECO:0000305|PubMed:24303041, ECO:0000305|PubMed:26211609

Chain	Residue	Details
A	ALA143
B	ALA143
C	ALA143
D	ALA143

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01339, ECO:0000269|PubMed:26211609

Chain	Residue	Details
A	HIS142
D	HIS142
D	HIS146
D	GLU185
A	HIS146
A	GLU185
B	HIS142
B	HIS146
B	GLU185
C	HIS142
C	HIS146
C	GLU185

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01339

Chain	Residue	Details
A	PHE178
B	PHE178
C	PHE178
D	PHE178

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Interacts with substrate peptide => ECO:0000269|PubMed:26211609

Chain	Residue	Details
A	ASP135
A	HIS142
B	ASP135
B	HIS142
C	ASP135
C	HIS142
D	ASP135
D	HIS142

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:26211609

Chain	Residue	Details
A	PHE178
B	PHE178
C	PHE178
D	PHE178

---