6R4Z
Crystal structure of holo PPEP-1(E143A/Y178F) in complex with product peptide Ac-EVNP-CO2 (substrate peptide: Ac-EVNPPVP-CONH2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Pro-Pro endopeptidase | polymer | 198 | 21909.6 | 2 | UniProt (Q183R7) Pfam (PF07737) | Peptoclostridium difficile | PPEP-1,Zinc metalloprotease Zmp1 |
| 2 | C, D (D, E) | ACE-GLU-VAL-ASN-PRO | polymer | 5 | 483.5 | 2 | Clostridioides difficile | ||
| 3 | E, G (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | F (A) | NICKEL (II) ION | non-polymer | 58.7 | 1 | Chemie (NI) | |||
| 5 | H, I, J, K (A, B, D, E) | water | water | 18.0 | 631 | Chemie (HOH) |
Sequence modifications
A, B: 27 - 220 (UniProt: Q183R7)
| PDB | External Database | Details |
|---|---|---|
| Gly 23 | - | expression tag |
| Ser 24 | - | expression tag |
| His 25 | - | expression tag |
| Met 26 | - | expression tag |
| Ala 143 | Glu 143 | conflict |
| Phe 178 | Tyr 178 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 44786.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 189.5 | |
| All* | Total formula weight | 44975.8 |
