6R4Z

Crystal structure of holo PPEP-1(E143A/Y178F) in complex with product peptide Ac-EVNP-CO2 (substrate peptide: Ac-EVNPPVP-CONH2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008237	molecular_function	metallopeptidase activity
A	0046872	molecular_function	metal ion binding
B	0005576	cellular_component	extracellular region
B	0006508	biological_process	proteolysis
B	0008237	molecular_function	metallopeptidase activity
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 301

Chain	Residue
A	HIS142
A	HIS146
A	GLU185
E	PRO4

---

site_id	AC2
Number of Residues	4
Details	binding site for residue NI A 302

Chain	Residue
A	GLY23
A	SER24
A	HIS25
A	MET26

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN B 300

Chain	Residue
B	HIS146
B	GLU185
D	PRO4
B	HIS142

---

site_id	AC4
Number of Residues	12
Details	binding site for Di-peptide ACE D 0 and GLU D 1

Chain	Residue
B	THR30
B	GLN32
B	GLY118
B	SER119
B	HIS150
B	HOH451
D	VAL2
D	ASN3
D	HOH101
D	HOH102
D	HOH103
D	HOH104

---

site_id	AC5
Number of Residues	10
Details	binding site for Di-peptide ACE E 0 and GLU E 1

Chain	Residue
A	GLY118
A	SER119
A	HIS150
A	LYS158
A	GLN198
A	HOH474
A	HOH731
E	VAL2
E	ASN3
E	HOH101

---

site_id	AC6
Number of Residues	10
Details	binding site for Di-peptide ACE E 0 and GLU E 1

Chain	Residue
A	GLY118
A	SER119
A	HIS150
A	LYS158
A	GLN198
A	HOH474
A	HOH731
E	VAL2
E	ASN3
E	HOH101

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU01339, ECO:0000305|PubMed:24303041, ECO:0000305|PubMed:26211609

Chain	Residue	Details
A	ALA143
B	ALA143

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01339, ECO:0000269|PubMed:26211609

Chain	Residue	Details
A	HIS142
A	HIS146
A	GLU185
B	HIS142
B	HIS146
B	GLU185

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01339

Chain	Residue	Details
A	PHE178
B	PHE178

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Interacts with substrate peptide => ECO:0000269|PubMed:26211609

Chain	Residue	Details
A	ASP135
A	HIS142
B	ASP135
B	HIS142

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:26211609

Chain	Residue	Details
A	PHE178
B	PHE178

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