6QY3

Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	CRISPR-associated protein Csn2	polymer	219	25533.5	16	UniProt (G3ECR4) Pfam (PF09711) In PDB	Streptococcus thermophilus
2	I, J, L, M, O...	CRISPR-associated endonuclease Cas1	polymer	302	35363.5	24	UniProt (G3ECR2) Pfam (PF01867) In PDB	Streptococcus thermophilus
3	K, N, Q, T, W...	CRISPR-associated endoribonuclease Cas2	polymer	114	13431.6	12	UniProt (G3ECR3) Pfam (PF09827) In PDB	Streptococcus thermophilus
4	1	DNA (66-MER)	polymer	66	20031.8	1		Escherichia coli BL21(DE3)
5	2	DNA (66-MER)	polymer	66	20626.7	1		Escherichia coli BL21(DE3)
6	A, B, E, F, a...	CALCIUM ION	non-polymer		40.1	16	Chemie (CA)

Sequence modifications

I, J, L, M, O, P, R, S, U, V, Y, Z, i, j, l, m, o, p, r, s, u, v, y, z: 1 - 289 (UniProt: G3ECR2)

PDB	External Database	Details
Leu 290	-	expression tag
Glu 291	-	expression tag
Ser 292	-	expression tag
Gly 293	-	expression tag
Trp 294	-	expression tag
Ser 295	-	expression tag
His 296	-	expression tag
Pro 297	-	expression tag
Gln 298	-	expression tag
Phe 299	-	expression tag
Glu 300	-	expression tag
Lys 301	-	expression tag
Ala 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	54
	Total formula weight	1459095.8
Non-Polymers*	Number of molecules	16
	Total formula weight	641.2
All*	Total formula weight	1459737.0

*Water molecules are not included.