6QXF
Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | CRISPR-associated protein Csn2 | polymer | 219 | 25533.5 | 8 | UniProt (G3ECR4) Pfam (PF09711) | Streptococcus thermophilus | |
| 2 | I, J, L, M, O... (I, J, L, M, O...) | CRISPR-associated endonuclease Cas1 | polymer | 302 | 35363.5 | 8 | UniProt (G3ECR2) Pfam (PF01867) | Streptococcus thermophilus | |
| 3 | K, N, Q, T (K, N, Q, T) | CRISPR-associated endoribonuclease Cas2 | polymer | 114 | 13431.6 | 4 | UniProt (G3ECR3) Pfam (PF09827) | Streptococcus thermophilus | |
| 4 | U (X) | DNA (25-MER) | polymer | 25 | 7559.9 | 1 | Escherichia coli BL21(DE3) | ||
| 5 | V (Y) | DNA (25-MER) | polymer | 25 | 7785.2 | 1 | Escherichia coli BL21(DE3) | ||
| 6 | AA, BA, CA, DA, W... (E, F, G, H, A...) | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) |
Sequence modifications
I, J, L, M, O, P, R, S: 1 - 289 (UniProt: G3ECR2)
| PDB | External Database | Details |
|---|---|---|
| Leu 290 | - | expression tag |
| Glu 291 | - | expression tag |
| Ser 292 | - | expression tag |
| Gly 293 | - | expression tag |
| Trp 294 | - | expression tag |
| Ser 295 | - | expression tag |
| His 296 | - | expression tag |
| Pro 297 | - | expression tag |
| Gln 298 | - | expression tag |
| Phe 299 | - | expression tag |
| Glu 300 | - | expression tag |
| Lys 301 | - | expression tag |
| Ala 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 22 |
| Total formula weight | 556246.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 320.6 | |
| All* | Total formula weight | 556567.4 |
