6QFM

Structure of human Mcl-1 in complex with PUMA BH3 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Induced myeloid leukemia cell differentiation protein Mcl-1	polymer	162	18352.9	1	UniProt (Q07820) Pfam (PF00452) In PDB	Homo sapiens (Human)	Bcl-2-like protein 3,Bcl2-L-3,Bcl-2-related protein EAT/mcl1,mcl1/EAT
2	B	Bcl-2-binding component 3	polymer	23	2748.0	1	UniProt (Q9BXH1) In PDB	Homo sapiens	JFY-1,p53 up-regulated modulator of apoptosis
3	A	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
4	A, B	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5		water	water		18.0	83	Chemie (HOH)

Sequence modifications

A: 171 - 327 (UniProt: Q07820)

PDB	External Database	Details
Gly 166	-	expression tag
Pro 167	-	expression tag
Leu 168	-	expression tag
Gly 169	-	expression tag
Ser 170	-	expression tag
Asp 173	Glu 173	engineered mutation
Gly 241	Asp 241	engineered mutation
Phe 246	Leu 246	engineered mutation
Val 251	Ile 251	engineered mutation
Lys 255	Ser 255	engineered mutation
Ser 280	Thr 280	engineered mutation
Val 281	Ile 281	engineered mutation
Phe 286	Cys 286	engineered mutation
Thr 293	Ser 293	engineered mutation
Gln 322	Glu 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	21100.8
Non-Polymers*	Number of molecules	6
	Total formula weight	302.6
All*	Total formula weight	21403.4

*Water molecules are not included.