6QBB

Engineered streptavidin variant (ENAGY) in complex with the Strep-tag II peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Streptavidin	polymer	127	13346.4	2	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	P, Q	Strep-tag II peptide	polymer	12	1574.1	2		synthetic construct
3		water	water		18.0	228	Chemie (HOH)

Sequence modifications

A, B: 14 - 139 (UniProt: P22629)

PDB	External Database	Details
Met 13	-	initiating methionine
Val 44	Glu 68	engineered mutation
Thr 45	Ser 69	engineered mutation
Arg 47	Val 71	engineered mutation
Glu 117	Ala 141	engineered mutation
Gly 120	Trp 144	engineered mutation
Tyr 121	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	29841.1
All*	Total formula weight	29841.1

*Water molecules are not included.