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6QBB

Engineered streptavidin variant (ENAGY) in complex with the Strep-tag II peptide

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.2
Synchrotron siteBESSY
Beamline14.2
Temperature [K]100
Detector technologyCCD
Collection date2013-12-12
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.91841
Spacegroup nameP 2 21 21
Unit cell lengths45.560, 64.170, 78.080
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.580 - 1.520
R-factor0.16295
Rwork0.162
R-free0.19021
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1rst
RMSD bond length0.011
RMSD bond angle1.483
Data reduction softwareMOSFLM (7.1.0)
Data scaling softwareSCALA (3.3.21)
Phasing softwarePHASER (2.5.6)
Refinement softwareREFMAC (5.8.0073)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.5801.600
High resolution limit [Å]1.5201.520
Rmerge0.0760.342
Rmeas0.0850.386
Rpim0.0390.176
Number of reflections359925162
<I/σ(I)>11.1
Completeness [%]100.099.8
Redundancy4.74.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.5293PEG MME 550, zinc sulfate, 2-(N-morpholino)ethanesulfonic acid

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