6Q9R

Crystal structure of the pathological N184K variant of calcium-free human gelsolin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Gelsolin	polymer	778	85532.8	2	UniProt (P06396) Pfam (PF00626) In PDB	Homo sapiens (Human)	AGEL,Actin-depolymerizing factor,ADF,Brevin
2	A, B	CHLORIDE ION	non-polymer		35.5	9	Chemie (CL)
3	B, A	GLYCEROL	non-polymer		92.1	17	Chemie (GOL)
4	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	B	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
6		water	water		18.0	232	Chemie (HOH)

Sequence modifications

A, B: 1 - 755 (UniProt: P06396)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Lys 184	Asn 211	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	171065.5
Non-Polymers*	Number of molecules	29
	Total formula weight	2198.9
All*	Total formula weight	173264.5

*Water molecules are not included.