6Q9J

Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	NADH-quinone oxidoreductase subunit E	polymer	160	18603.6	2	UniProt (O66842) Pfam (PF01257)	Aquifex aeolicus (strain VF5)	NADH dehydrogenase I subunit E,NDH-1 subunit E
2	B, D (B, D)	NADH-quinone oxidoreductase subunit F	polymer	434	48523.3	2	UniProt (O66841) Pfam (PF01512) Pfam (PF22461) Pfam (PF10531) Pfam (PF10589)	Aquifex aeolicus (strain VF5)	NADH dehydrogenase I subunit F,NDH-1 subunit F
3	E, O (A, C)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
4	F, G, Q, R, Y (A, C, D)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	H, S (B, D)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
6	I, T (B, D)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
7	J, U (B, D)	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	2	Chemie (NAI)
8	K, L, P, V, W... (B, C, D)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
9	AA, M, N, Z (D, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
10	BA, CA, DA, EA (A, B, C, D)	water	water		18.0	977	Chemie (HOH)

Sequence modifications

A, C: 1 - 160 (UniProt: O66842)

PDB	External Database	Details
Ser 129	Gly 129	engineered mutation

B, D: 1 - 426 (UniProt: O66841)

PDB	External Database	Details
Ala 427	-	expression tag
Gly 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134253.9
Non-Polymers*	Number of molecules	23
	Total formula weight	4423.3
All*	Total formula weight	138677.2

*Water molecules are not included.