6Q2S

Cryo-EM structure of RET/GFRa3/ARTN extracellular complex. The 3D refinement was applied with C2 symmetry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Ubiquitin-like protein SMT3,Artemin	polymer	235	25793.3	2	UniProt (Q12306) UniProt (Q5T4W7) Pfam (PF00240) Pfam (PF00019)	Saccharomyces cerevisiae (Baker's yeast)	Enovin,Neublastin
2	B, E (C, D)	GDNF family receptor alpha-3	polymer	342	38370.0	2	UniProt (O60609) Pfam (PF02351)	Homo sapiens (Human)	GFR-alpha-3
3	C, F (E, F)	Proto-oncogene tyrosine-protein kinase receptor Ret	polymer	617	69100.8	2	UniProt (P07949) Pfam (PF22540) Pfam (PF17756) Pfam (PF00028) Pfam (PF17812) Pfam (PF17813)	Homo sapiens (Human)	Cadherin family member 12,Proto-oncogene c-Ret
4	G, H, I, J, K... (C, E, D, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	14	Chemie (NAG)
5	AA, BA, N, O, P... (F, E)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)

Sequence modifications

A, B: 9 - 106 (UniProt: Q12306)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ser -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Val 0	-	expression tag
Pro 1	-	expression tag
Arg 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Met 6	-	expression tag
Ala 7	-	expression tag
Ser 8	-	expression tag
Ser 107	-	linker

A, B: 108 - 220 (UniProt: Q5T4W7)
C, D: 32 - 363 (UniProt: O60609)

PDB	External Database	Details
Gly 364	-	expression tag
Thr 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

E, F: 29 - 635 (UniProt: P07949)

PDB	External Database	Details
His 114	Arg 114	conflict
Gly 636	-	expression tag
Thr 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	266528.2
Non-Polymers*	Number of molecules	22
	Total formula weight	3417.5
All*	Total formula weight	269945.8

*Water molecules are not included.