6Q2R

Cryo-EM structure of RET/GFRa2/NRTN extracellular complex in the tetrameric form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H (A, B, U, V)	Neurturin	polymer	102	11706.4	4	UniProt (Q99748) Pfam (PF00019)	Homo sapiens (Human)
2	C, E, I, K (C, D, W, X)	GDNF family receptor alpha-2	polymer	349	39572.6	4	UniProt (O00451) Pfam (PF02351)	Homo sapiens (Human)	GFR-alpha-2,GDNF receptor beta,GDNFR-beta,Neurturin receptor alpha,NTNR-alpha,RET ligand 2,TGF-beta-related neurotrophic factor receptor 2
3	D, F, J, L (E, F, Y, Z)	Proto-oncogene tyrosine-protein kinase receptor Ret	polymer	617	69100.8	4	UniProt (P07949) Pfam (PF17756) Pfam (PF00028) Pfam (PF17812) Pfam (PF17813) Pfam (PF22540)	Homo sapiens (Human)	Cadherin family member 12,Proto-oncogene c-Ret
4	GA, HA, IA, JA, M... (Y, E, Z, F)	CALCIUM ION	non-polymer		40.1	16	Chemie (CA)
5	AA, BA, CA, DA, EA... (F, Y, E, Z)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	24	Chemie (NAG)

Sequence modifications

C, D, W, X: 24 - 362 (UniProt: O00451)

PDB	External Database	Details
Gly 363	-	expression tag
Thr 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag

E, F, Y, Z: 29 - 635 (UniProt: P07949)

PDB	External Database	Details
His 114	Arg 114	conflict
Gly 636	-	expression tag
Thr 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	481519.4
Non-Polymers*	Number of molecules	40
	Total formula weight	5950.2
All*	Total formula weight	487469.6

*Water molecules are not included.