6PWQ

Crystal structure of Levansucrase from Bacillus subtilis mutant S164A at 2.6 A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycoside hydrolase family 68 protein	polymer	466	52446.8	2	UniProt (A0PFL2) Pfam (PF02435) UniProt (by SIFTS) (P05655)	Bacillus subtilis	Levansucrase
2	C, Q (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	AA, D, E, F, G... (B, A)	SULFATE ION	non-polymer		96.1	20	Chemie (SO4)
4	BA, N (B, A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
5	CA, DA, EA, O, P (B, A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	FA, GA (A, B)	water	water		18.0	187	Chemie (HOH)

Sequence modifications

A, B: 30 - 473 (UniProt: A0PFL2)

PDB	External Database	Details
Ala 164	Ser 164	engineered mutation
Thr 474	-	expression tag
Asp 475	-	expression tag
Pro 476	-	expression tag
Asn 477	-	expression tag
Ser 478	-	expression tag
Ser 479	-	expression tag
Ser 480	-	expression tag
Val 481	-	expression tag
Asp 482	-	expression tag
Lys 483	-	expression tag
Leu 484	-	expression tag
Ala 485	-	expression tag
Ala 486	-	expression tag
Ala 487	-	expression tag
Leu 488	-	expression tag
Glu 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	104893.7
Non-Polymers*	Number of molecules	29
	Total formula weight	2850.3
All*	Total formula weight	107744.0

*Water molecules are not included.