6PSK

Crystal structure of the complex between periplasmic domains of antiholin RI and holin T from T4 phage, in P6522

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Antiholin	polymer	74	9083.3	1	UniProt (I7AU04) UniProt (by SIFTS) (P13304) In PDB	Escherichia phage ECML-134
2	T	Holin	polymer	155	18102.9	1	UniProt (A0A2U8QQK7) Pfam (PF11031) UniProt (by SIFTS) (P06808) In PDB	Escherichia phage vB_EcoM_NBG2
3	R, T	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	R, T	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	T	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	1	Chemie (BTB)
6	T	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7		water	water		18.0	73	Chemie (HOH)

Sequence modifications

R: 25 - 97 (UniProt: I7AU04)

PDB	External Database	Details
Mse 24	-	initiating methionine

T: 77 - 218 (UniProt: A0A2U8QQK7)

PDB	External Database	Details
Lys 219	-	expression tag
Leu 220	-	expression tag
Ala 221	-	expression tag
Ala 222	-	expression tag
Ala 223	-	expression tag
Leu 224	-	expression tag
Glu 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27186.2
Non-Polymers*	Number of molecules	9
	Total formula weight	781.2
All*	Total formula weight	27967.4

*Water molecules are not included.