6PSK
Crystal structure of the complex between periplasmic domains of antiholin RI and holin T from T4 phage, in P6522
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-25 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 120.666, 120.666, 85.513 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.586 - 2.200 |
| R-factor | 0.2144 |
| Rwork | 0.213 |
| R-free | 0.24680 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.893 |
| Data reduction software | TRUNCATE |
| Data scaling software | SAINT |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.790 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.700 | 2.200 |
| Number of reflections | 19177 | 969 | 734 |
| <I/σ(I)> | 16.17 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 40.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 1.0M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, and 1% PEG 3,350 |
