6PP9

Crystal structure of BRAF:MEK1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Serine/threonine-protein kinase B-raf	polymer	283	32098.1	1	UniProt (P15056) Pfam (PF07714)	Homo sapiens (Human)	Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1
2	B (B)	Dual specificity mitogen-activated protein kinase kinase 1	polymer	397	43790.3	1	UniProt (Q02750) Pfam (PF00069)	Homo sapiens (Human)	MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1
3	C, F (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
4	D, G (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	E, J (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	H (B)	5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide	non-polymer		456.2	1	Chemie (LCJ)
7	I (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
8	K, L (B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
9	M, N (A, B)	water	water		18.0	72	Chemie (HOH)

Sequence modifications

A: 445 - 723 (UniProt: P15056)

PDB	External Database	Details
Gly 441	-	expression tag
Gly 442	-	expression tag
Gly 443	-	expression tag
Arg 444	-	expression tag

B: 1 - 393 (UniProt: Q02750)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ala 218	Ser 218	engineered mutation
Ala 222	Ser 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75888.4
Non-Polymers*	Number of molecules	10
	Total formula weight	1868.4
All*	Total formula weight	77756.8

*Water molecules are not included.