6PHY

SpAga D472N structure in complex alpha 1,3 galactobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-galactosidase	polymer	740	83967.2	1	UniProt (A0A0H2URQ6) Pfam (PF16875) Pfam (PF02065) Pfam (PF16874) In PDB	Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
2	B	alpha-D-galactopyranose-(1-3)-beta-D-galactopyranose	branched		342.3	1	In PDB GlyTouCan (G24432YB)
3	C	alpha-D-galactopyranose-(1-3)-alpha-D-galactopyranose	branched		342.3	1	In PDB GlyTouCan (G91563HD)
4	A	L(+)-TARTARIC ACID	non-polymer		150.1	2	Chemie (TLA)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
6		water	water		18.0	575	Chemie (HOH)

Sequence modifications

A: 1 - 720 (UniProt: A0A0H2URQ6)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 472	Asp 472	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	83967.2
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	8
	Total formula weight	672.6
All*	Total formula weight	85324.4

*Water molecules are not included.