6PFJ

Structure of S. venezuelae RsiG-WhiG-(ci-di-GMP) complex, P64 crystal form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	T	AmfC protein	polymer	176	19992.4	1	UniProt (F2RFR7) Pfam (PF22802) In PDB	Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)	RsiG
2	A	RNA polymerase sigma factor	polymer	278	30924.0	1	UniProt (A0A3N1Q704) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Streptomyces sp. PanSC19	WhiG
3	T	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	2	Chemie (C2E)
4		water	water		18.0	43	Chemie (HOH)

Sequence modifications

T: 26 - 199 (UniProt: F2RFR7)

PDB	External Database	Details
Gly 24	-	expression tag
Ser 25	-	expression tag
Gly 91	Pro 91	engineered mutation

A: 1 - 278 (UniProt: A0A3N1Q704)

PDB	External Database	Details
Glu 38	Asp 38	engineered mutation
Thr 150	Ser 150	engineered mutation
Ser 159	Thr 159	engineered mutation
Asp 162	Glu 162	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50916.4
Non-Polymers*	Number of molecules	2
	Total formula weight	1380.8
All*	Total formula weight	52297.2

*Water molecules are not included.