6PFJ
Structure of S. venezuelae RsiG-WhiG-(ci-di-GMP) complex, P64 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 64 |
Unit cell lengths | 92.089, 92.089, 96.646 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 79.750 - 2.080 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.277 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AutoSol (autosol) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.800 | 2.110 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.051 | 0.869 |
Number of reflections | 53003 | |
<I/σ(I)> | 20.5 | 1.4 |
Completeness [%] | 96.0 | 94 |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris Bicine pH 8.5, 0.03 M sodium nitrate, 0.03 M sodium phosphate, 0.03 M ammonium sulfate, 9% MPD, 10% PEG 1000, 15% PEG 3350 |