6PCC
Crystal structure of beta-ketoadipyl-CoA thiolase mutant (H356A) in complex hexanoyl coenzyme A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-ketoadipyl-CoA thiolase | polymer | 423 | 44452.6 | 4 | UniProt (Q88N39) Pfam (PF00108) Pfam (PF02803) | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) | |
| 2 | E, H, K, N (A, B, C, D) | COENZYME A | non-polymer | 767.5 | 4 | Chemie (COA) | |||
| 3 | F, I, L, O (A, B, C, D) | hexanal | non-polymer | 100.2 | 4 | Chemie (O8Y) | |||
| 4 | G, J, M, P (A, B, C, D) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 5 | Q, R, S, T (A, B, C, D) | water | water | 18.0 | 1215 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 400 (UniProt: Q88N39)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Ser -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Val -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Thr -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| His -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 356 | His 356 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 177810.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 3839.1 | |
| All* | Total formula weight | 181649.7 |
