6PB0
Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 1 receptor in complex with Gs protein and Nb35
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Corticotropin-releasing factor receptor 1 | polymer | 375 | 43509.7 | 1 | UniProt (P34998) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | CRFR-1,Corticotropin-releasing hormone receptor 1,CRH-R1 |
| 2 | B (U) | Urocortin | polymer | 40 | 4703.3 | 1 | UniProt (P55089) Pfam (PF00473) | Homo sapiens (Human) | |
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 378 | 43897.8 | 1 | UniProt (P63092) UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 6 | F (N) | Nanobody 35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
| 7 | G, H, I, J, L (R) | CHOLESTEROL | non-polymer | 386.7 | 5 | Chemie (CLR) | |||
| 8 | K, M, N, O, P... (R) | PALMITIC ACID | non-polymer | 256.4 | 7 | Chemie (PLM) |
Sequence modifications
A: 1 - 83 (UniProt: P63092)
A: 84 - 205 (UniProt: P63096)
A: 206 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
A: 84 - 205 (UniProt: P63096)
A: 206 - 394 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 226 | Gly 226 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 153230.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 3728.2 | |
| All* | Total formula weight | 156958.7 |
