Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6PB0

Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 1 receptor in complex with Gs protein and Nb35

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0019001	molecular_function	guanyl nucleotide binding
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0001750	cellular_component	photoreceptor outer segment
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005765	cellular_component	lysosomal membrane
B	0005829	cellular_component	cytosol
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0005886	cellular_component	plasma membrane
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
B	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
B	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
B	0007265	biological_process	Ras protein signal transduction
B	0008283	biological_process	cell population proliferation
B	0016020	cellular_component	membrane
B	0030159	molecular_function	signaling receptor complex adaptor activity
B	0044877	molecular_function	protein-containing complex binding
B	0045202	cellular_component	synapse
B	0050909	biological_process	sensory perception of taste
B	0051020	molecular_function	GTPase binding
B	0060041	biological_process	retina development in camera-type eye
B	0070062	cellular_component	extracellular exosome
B	0071380	biological_process	cellular response to prostaglandin E stimulus
B	0071870	biological_process	cellular response to catecholamine stimulus
B	0097381	cellular_component	photoreceptor disc membrane
B	1903561	cellular_component	extracellular vesicle
G	0005515	molecular_function	protein binding
G	0005834	cellular_component	heterotrimeric G-protein complex
G	0005886	cellular_component	plasma membrane
G	0007165	biological_process	signal transduction
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
G	0016020	cellular_component	membrane
G	0031681	molecular_function	G-protein beta-subunit binding
G	0048144	biological_process	fibroblast proliferation
G	0070062	cellular_component	extracellular exosome
G	0071380	biological_process	cellular response to prostaglandin E stimulus
G	0071870	biological_process	cellular response to catecholamine stimulus
R	0004888	molecular_function	transmembrane signaling receptor activity
R	0004930	molecular_function	G protein-coupled receptor activity
R	0005515	molecular_function	protein binding
R	0005768	cellular_component	endosome
R	0005886	cellular_component	plasma membrane
R	0006955	biological_process	immune response
R	0007166	biological_process	cell surface receptor signaling pathway
R	0007186	biological_process	G protein-coupled receptor signaling pathway
R	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
R	0007190	biological_process	activation of adenylate cyclase activity
R	0007565	biological_process	female pregnancy
R	0007567	biological_process	parturition
R	0008528	molecular_function	G protein-coupled peptide receptor activity
R	0010578	biological_process	regulation of adenylate cyclase activity involved in G protein-coupled receptor signaling pathway
R	0015056	molecular_function	corticotrophin-releasing factor receptor activity
R	0016020	cellular_component	membrane
R	0030325	biological_process	adrenal gland development
R	0035640	biological_process	exploration behavior
R	0042596	biological_process	fear response
R	0043005	cellular_component	neuron projection
R	0048149	biological_process	behavioral response to ethanol
R	0051424	molecular_function	corticotropin-releasing hormone binding
R	0051458	biological_process	corticotropin secretion
R	0051867	biological_process	general adaptation syndrome, behavioral process
R	0071376	biological_process	cellular response to corticotropin-releasing hormone stimulus
R	1901386	biological_process	negative regulation of voltage-gated calcium channel activity
R	2000852	biological_process	regulation of corticosterone secretion
U	0005179	molecular_function	hormone activity
U	0005576	cellular_component	extracellular region

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue CLR R 401

Chain	Residue
R	THR168
R	THR175

site_id	AC2
Number of Residues	2
Details	binding site for residue CLR R 402

Chain	Residue
R	ARG143
R	ARG377

site_id	AC3
Number of Residues	2
Details	binding site for residue CLR R 403

Chain	Residue
R	VAL339
R	PHE350

site_id	AC4
Number of Residues	2
Details	binding site for residue CLR R 404

Chain	Residue
R	ARG189
R	TRP259

site_id	AC5
Number of Residues	1
Details	binding site for residue PLM R 405

Chain	Residue
R	HIS115

site_id	AC6
Number of Residues	2
Details	binding site for residue CLR R 406

Chain	Residue
R	TRP236
R	PLM409

site_id	AC7
Number of Residues	1
Details	binding site for residue PLM R 407

Chain	Residue
R	PHE365

site_id	AC8
Number of Residues	2
Details	binding site for residue PLM R 408

Chain	Residue
R	TYR253
R	TYR267

site_id	AC9
Number of Residues	5
Details	binding site for residue PLM R 409

Chain	Residue
R	TRP156
R	ASN157
R	SER160
R	LYS228
R	CLR406

site_id	AD1
Number of Residues	2
Details	binding site for residue PLM R 411

Chain	Residue
R	TYR267
R	GLY274

Functional Information from PROSITE/UniProt

site_id	PS00511
Number of Residues	16
Details	CRF Corticotropin-releasing factor family signature. PsLSIdlTFhLLRtlL

Chain	Residue	Details
U	PRO3-LEU18

site_id	PS00649
Number of Residues	26
Details	G_PROTEIN_RECEP_F2_1 G-protein coupled receptors family 2 signature 1. CnasVDligtCWprSpagqlvvrpCP

Chain	Residue	Details
R	CYS44-PRO69

site_id	PS00650
Number of Residues	16
Details	G_PROTEIN_RECEP_F2_2 G-protein coupled receptors family 2 signature 2. QGFFVsVFYCFlNseV

Chain	Residue	Details
R	GLN355-VAL370

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
B	LEU70-SER84
B	ILE157-ILE171
B	LEU285-ALA299

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:22814378

Chain	Residue	Details
G	ALA2
A	SER54
A	ASP223
A	ASN292
A	SER366

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Cysteine methyl ester => ECO:0000250\|UniProtKB:P63212

Chain	Residue	Details
G	CYS68

site_id	SWS_FT_FI3
Number of Residues	1
Details	LIPID: S-geranylgeranyl cysteine => ECO:0000250\|UniProtKB:P63212

Chain	Residue	Details
G	CYS68
R	LEU219-ARG225
R	THR296-THR306

site_id	SWS_FT_FI4
Number of Residues	1
Details	LIPID: S-palmitoyl cysteine => ECO:0000269\|PubMed:21044946

Chain	Residue	Details
A	CYS3

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
A	LYS300

site_id	SWS_FT_FI6
Number of Residues	1
Details	BINDING: BINDING => ECO:0007744\|PDB:1KJY, ECO:0007744\|PDB:2OM2, ECO:0007744\|PDB:2XNS, ECO:0007744\|PDB:3ONW, ECO:0007744\|PDB:3QE0, ECO:0007744\|PDB:3QI2, ECO:0007744\|PDB:4G5Q

Chain	Residue	Details
A	SER174

site_id	SWS_FT_FI7
Number of Residues	1
Details	BINDING: BINDING => ECO:0007744\|PDB:1KJY, ECO:0007744\|PDB:1Y3A, ECO:0007744\|PDB:2G83, ECO:0007744\|PDB:2GTP, ECO:0007744\|PDB:2IK8, ECO:0007744\|PDB:2OM2, ECO:0007744\|PDB:2XNS, ECO:0007744\|PDB:3ONW, ECO:0007744\|PDB:3QE0, ECO:0007744\|PDB:3QI2, ECO:0007744\|PDB:3UMR, ECO:0007744\|PDB:3UMS, ECO:0007744\|PDB:4G5Q

Chain	Residue	Details
A	LEU198

site_id	SWS_FT_FI8
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:18434541, ECO:0000269\|PubMed:22383884, ECO:0007744\|PDB:3QE0

Chain	Residue	Details
A	THR204

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250

Chain	Residue	Details
A	ARG201

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269\|PubMed:14657255

Chain	Residue	Details
R	SER301

site_id	SWS_FT_FI11
Number of Residues	5
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
R	ASN38
R	ASN45
R	ASN78
R	ASN90
R	ASN98

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)