6P9X

CRF1 Receptor Gs GPCR protein complex with CRF1 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45700.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (N)	Nanobody 35	polymer	138	15140.7	1		Lama glama (llama)
5	E (R)	Corticotropin-releasing factor receptor 1	polymer	427	49392.1	1	UniProt (P34998) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (Human)	CRFR-1,Corticotropin-releasing hormone receptor 1,CRH-R1
6	F (P)	Corticoliberin	polymer	41	4764.5	1	UniProt (P06850) Pfam (PF00473)	Homo sapiens	Corticotropin-releasing factor,CRF,Corticotropin-releasing hormone
7	G, H, I (A, R, P)	water	water		18.0	17	Chemie (HOH)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 23 - 415 (UniProt: P34998)

PDB	External Database	Details
Asp 8	-	expression tag
Tyr 9	-	expression tag
Lys 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Leu 15	-	expression tag
Glu 16	-	expression tag
Val 17	-	expression tag
Leu 18	-	expression tag
Phe 19	-	expression tag
Gln 20	-	expression tag
Gly 21	-	expression tag
Pro 22	-	expression tag
Thr 222	Ser 222	conflict
Pro 416	-	expression tag
Ala 417	-	expression tag
Gly 418	-	expression tag
Leu 419	-	expression tag
Glu 420	-	expression tag
Val 421	-	expression tag
Leu 422	-	expression tag
Phe 423	-	expression tag
Gln 424	-	expression tag
Gly 425	-	expression tag
Pro 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	161393.0
All*	Total formula weight	161393.0

*Water molecules are not included.