6P9V

Crystal Structure of hMAT Mutant K289L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	S-adenosylmethionine synthase isoform type-2	polymer	415	45875.9	1	UniProt (P31153) Pfam (PF00438) Pfam (PF02772) Pfam (PF02773) In PDB	Homo sapiens (Human)	AdoMet synthase 2,Methionine adenosyltransferase 2,MAT 2,Methionine adenosyltransferase II,MAT-II
2	A	ADENOSINE	non-polymer		267.2	1	Chemie (ADN)
3	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
6		water	water		18.0	158	Chemie (HOH)

Sequence modifications

A: 1 - 395 (UniProt: P31153)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 289	Lys 289	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45875.9
Non-Polymers*	Number of molecules	4
	Total formula weight	506.6
All*	Total formula weight	46382.6

*Water molecules are not included.