6P9U

Crystal structure of human thrombin mutant W215A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Prothrombin	polymer	31	3647.1	4	UniProt (P00734) Pfam (PF09396)	Homo sapiens (Human)	Coagulation factor II
2	B, D, F, H (B, D, F, H)	Prothrombin	polymer	273	31309.9	4	UniProt (P00734) Pfam (PF00089)	Homo sapiens (Human)	Coagulation factor II
3	I, K, L, M, O... (B, C, D, E, F...)	ZINC ION	non-polymer		65.4	7	Chemie (ZN)
4	J, N, Q, S (B, D, F, H)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	T, U, V, W, X (B, D, E, F, H)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

B, D, F, H: 16 - 247 (UniProt: P00734)

PDB	External Database	Details
Ala 215	Trp 590	engineered mutation
Tyr 248	-	expression tag
Leu 249	-	expression tag
Glu 250	-	expression tag
Asp 251	-	expression tag
Gln 252	-	expression tag
Val 253	-	expression tag
Asp 254	-	expression tag
Pro 255	-	expression tag
Arg 256	-	expression tag
Leu 257	-	expression tag
Ile 258	-	expression tag
Asp 259	-	expression tag
Gly 260	-	expression tag
Lys 261	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	139827.9
Non-Polymers*	Number of molecules	11
	Total formula weight	1342.7
All*	Total formula weight	141170.6

*Water molecules are not included.