6P81

Structure of DNA polymerase III, beta subunit/ beta sliding clamp from Klebsiella pneumoniae, expressed with an N-terminal His-Smt3 fusion tag, in complex with Griselimycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-like protein SMT3,Beta sliding clamp	polymer	462	51735.0	1	UniProt (Q12306) UniProt (W1BGQ6) Pfam (PF00240)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B (B)	Griselimycin	polymer	11	1115.4	1	BIRD (PRD_002311)	Streptomyces caelicus
3	C (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	D (A)	1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE	non-polymer		178.2	1	Chemie (PG5)
5	E (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	G, H (A, B)	water	water		18.0	398	Chemie (HOH)

Sequence modifications

A: -85 - 0 (UniProt: Q12306)

PDB	External Database	Details
Met -95	-	initiating methionine
Gly -94	-	expression tag
His -93	-	expression tag
His -92	-	expression tag
His -91	-	expression tag
His -90	-	expression tag
His -89	-	expression tag
His -88	-	expression tag
Ser -87	-	expression tag
Gly -86	-	expression tag

A: 1 - 366 (UniProt: W1BGQ6)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52850.4
Non-Polymers*	Number of molecules	4
	Total formula weight	312.8
All*	Total formula weight	53163.2

*Water molecules are not included.