6P81
Structure of DNA polymerase III, beta subunit/ beta sliding clamp from Klebsiella pneumoniae, expressed with an N-terminal His-Smt3 fusion tag, in complex with Griselimycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-05-30 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 71.020, 83.100, 188.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.014 - 1.750 |
R-factor | 0.1858 |
Rwork | 0.185 |
R-free | 0.21260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 3dlg and |
RMSD bond length | 0.006 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.014 | 47.014 | 1.800 |
High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
Rmerge | 0.057 | 0.045 | 0.616 |
Rmeas | 0.062 | 0.049 | 0.663 |
Total number of observations | 417678 | ||
Number of reflections | 56497 | 699 | 4119 |
<I/σ(I)> | 18 | 35.9 | 3.14 |
Completeness [%] | 99.9 | 97.1 | 100 |
Redundancy | 7.393 | 6.117 | 7.422 |
CC(1/2) | 0.999 | 0.996 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 290 | Microlytic MCSG-2, condition D4: 30% (V/V) PEG 300, 200mM Calcium acetate, 100mM sodium acetate / acetic acid pH 4.5: KlpnA.17987.a.EN11.PD383542 at 20.95mg/ml + 2mM griselimycin: tray 309729 D4: cryo: direct: puck ECJ6-1. |