6P7E
Structure of T7 DNA Polymerase Bound to a Primer/Template DNA and a Peptide that Mimics the C-terminal Tail of the Primase-Helicase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | DNA-directed DNA polymerase | polymer | 704 | 79703.6 | 4 | UniProt (P00581) Pfam (PF00476) | Enterobacteria phage T7 (Bacteriophage T7) | Gene product 5,Gp5 |
| 2 | E, F, G, H (E, F, G, H) | TrxA | polymer | 109 | 11818.6 | 4 | UniProt (Q14F07) Pfam (PF00085) UniProt (by SIFTS) (P0AA25) | Escherichia coli | Thioredoxin 1 |
| 3 | I, K, M, O (I, K, M, O) | DNA (5'-D(P*GP*GP*CP*AP*GP*GP*TP*GP*GP*TP*CP*TP*TP*GP*CP*CP*GP*GP*TP*GP*A)-3') | polymer | 21 | 6551.2 | 4 | Enterobacteria phage T7 | ||
| 4 | J, L, N, P (J, L, N, P) | DNA (25-MER) | polymer | 25 | 7518.9 | 4 | Enterobacteria phage T7 | ||
| 5 | Q, R (U, V) | ASP-THR-ASP-PHE peptide | polymer | 4 | 496.5 | 2 | Enterobacteria phage T7 | ||
| 6 | S (W) | THR-ASP-PHE peptide | polymer | 3 | 381.4 | 1 | Enterobacteria phage T7 | ||
| 7 | CA, DA, HA, IA, MA... (B, C, D, A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 8 | Chemie (TTP) | |||
| 8 | AA, BA, EA, FA, GA... (B, C, D, A) | MAGNESIUM ION | non-polymer | 24.3 | 12 | Chemie (MG) | |||
| 9 | NA, OA, PA, QA, RA... (A, B, C, D, J...) | water | water | 18.0 | 31 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 704 (UniProt: P00581)
| PDB | External Database | Details |
|---|---|---|
| Ala 5 | Asp 5 | engineered mutation |
| Ala 7 | Glu 7 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 19 |
| Total formula weight | 423743.2 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 4149.0 | |
| All* | Total formula weight | 427892.2 |
