6P5C
Bacillus Fragment DNA polymerase mutant I716M
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | DNA (5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*C)-3') | polymer | 9 | 2700.8 | 1 | synthetic construct | ||
| 2 | B (C) | DNA (5'-D(P*GP*CP*GP*TP*GP*AP*TP*CP*G)-3') | polymer | 9 | 2771.8 | 1 | synthetic construct | ||
| 3 | C (A) | DNA polymerase I | polymer | 581 | 66322.1 | 1 | UniProt (D9N168) Pfam (PF22619) Pfam (PF00476) | Geobacillus stearothermophilus | |
| 4 | D, E, F, G (A) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 5 | H, I, J (B, C, A) | water | water | 18.0 | 144 | Chemie (HOH) |
Sequence modifications
A: 298 - 876 (UniProt: D9N168)
| PDB | External Database | Details |
|---|---|---|
| Met 296 | - | initiating methionine |
| Ala 297 | - | expression tag |
| Asp 598 | Ala 301 | conflict |
| Val 713 | Pro 416 | conflict |
| Met 716 | Ile 419 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 71794.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 384.3 | |
| All* | Total formula weight | 72178.9 |
