6P5C
Bacillus Fragment DNA polymerase mutant I716M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-06 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.217, 93.521, 105.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.340 - 2.200 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.27260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l3s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.524 |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 44306 | 4436 |
| <I/σ(I)> | 14.62 | 6.36 |
| Completeness [%] | 98.5 | 92.3 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 50% saturated ammonium sulfate, 100 mM MES, 2% methylpentanediol, 10 mM magnesium sulfate |
