6OXH

X-ray crystal structure of His-tagged Y140F FtmOx1 bound to Fe(II) and 2-oxoglutarate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Verruculogen synthase	polymer	313	35103.2	2	UniProt (Q4WAW9) Pfam (PF05721)	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus Af293)	Fumitremorgin biosynthesis protein F, FtmOx1
2	C, E (A, B)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
3	D, F (A, B)	2-OXOGLUTARIC ACID	non-polymer		146.1	2	Chemie (AKG)
4	G, H (A, B)	water	water		18.0	48	Chemie (HOH)

Sequence modifications

A, B: 1 - 291 (UniProt: Q4WAW9)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Phe 140	Tyr 140	engineered mutation
Leu 292	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70206.3
Non-Polymers*	Number of molecules	4
	Total formula weight	403.9
All*	Total formula weight	70610.2

*Water molecules are not included.