6OUN

Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and (-)3TC-TP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reverse transcriptase/ribonuclease H	polymer	558	64309.6	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Exoribonuclease H, p66 RT
2	B (B)	P51 RT	polymer	452	52748.4	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)
3	C (P)	DNA primer 20-mer	polymer	21	6460.2	1		synthetic construct
4	D (T)	DNA template 27-mer	polymer	27	8408.4	1		synthetic construct
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G, I, J (A, P, T)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
7	H (A)	Lamivudine Triphosphate	non-polymer		469.2	1	Chemie (1RZ)
8	K, L, M (A, B, T)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

A: 1 - 558 (UniProt: P03366)

PDB	External Database	Details
Arg 172	Lys 771	conflict
Cys 258	Gln 857	engineered mutation
Ser 280	Cys 879	engineered mutation
Arg 461	Lys 1060	conflict
Thr 468	Pro 1067	conflict
Asp 471	Asn 1070	conflict
Gln 512	Lys 1111	conflict

B: 1 - 440 (UniProt: P03366)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Arg 172	Lys 771	conflict
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	131926.7
Non-Polymers*	Number of molecules	6
	Total formula weight	806.0
All*	Total formula weight	132732.7

*Water molecules are not included.