6OT0
Structure of human Smoothened-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Smoothened homolog | polymer | 563 | 62109.4 | 1 | UniProt (Q99835) Pfam (PF01392) Pfam (PF01534) | Homo sapiens (Human) | SMO,Protein Gx |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (L) | Fab light chain | polymer | 215 | 23258.8 | 1 | Mus musculus (Mouse) | ||
| 6 | F (H) | Fab heavy chain | polymer | 239 | 25788.8 | 1 | Mus musculus (Mouse) | ||
| 7 | G (R) | 17-[3-(3,3-DIMETHYL-OXIRANYL)-1-METHYL-PROPYL]-10,13-DIMETHYL-2,3,4,7,8,9,10,11,12,13,14,15,16,17-TETRADECAHYDRO-1H-CYC LOPENTA[A]PHENANTHREN-3-OL | non-polymer | 400.6 | 1 | Chemie (CO1) |
Sequence modifications
R: 1 - 555 (UniProt: Q99835)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 556 | - | expression tag |
| Tyr 557 | - | expression tag |
| Lys 558 | - | expression tag |
| Asp 559 | - | expression tag |
| Asp 560 | - | expression tag |
| Asp 561 | - | expression tag |
| Asp 562 | - | expression tag |
| Lys 563 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | conflict |
| Ala 203 | Gly 203 | conflict |
| Ala 245 | Glu 245 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 198176.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 400.6 | |
| All* | Total formula weight | 198577.2 |
