6OT0
Structure of human Smoothened-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Smoothened homolog | polymer | 563 | 62109.4 | 1 | UniProt (Q99835) Pfam (PF01392) Pfam (PF01534) | Homo sapiens (Human) | SMO,Protein Gx |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (L) | Fab light chain | polymer | 215 | 23258.8 | 1 | Mus musculus (Mouse) | ||
| 6 | F (H) | Fab heavy chain | polymer | 239 | 25788.8 | 1 | Mus musculus (Mouse) | ||
| 7 | G (R) | 17-[3-(3,3-DIMETHYL-OXIRANYL)-1-METHYL-PROPYL]-10,13-DIMETHYL-2,3,4,7,8,9,10,11,12,13,14,15,16,17-TETRADECAHYDRO-1H-CYC LOPENTA[A]PHENANTHREN-3-OL | non-polymer | 400.6 | 1 | Chemie (CO1) PubChem (16759976) PubChem (44302258) PubChem (20610833) PubChem (50987447) PubChem (56947093) PubChem (3247059) PubChem (9887255) PubChem (10237666) PubChem (10250418) PubChem (15597289) PubChem (15597290) PubChem (16064712) PubChem (171318093) PubChem (126969898) PubChem (126969901) PubChem (142521819) PubChem (146159722) PubChem (162950489) PubChem (168322791) PubChem (169439820) PubChem (174841758) |
Sequence modifications
R: 1 - 555 (UniProt: Q99835)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 556 | - | expression tag |
| Tyr 557 | - | expression tag |
| Lys 558 | - | expression tag |
| Asp 559 | - | expression tag |
| Asp 560 | - | expression tag |
| Asp 561 | - | expression tag |
| Asp 562 | - | expression tag |
| Lys 563 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | conflict |
| Ala 203 | Gly 203 | conflict |
| Ala 245 | Glu 245 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 198176.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 400.6 | |
| All* | Total formula weight | 198577.2 |
