6OO3

Cryo-EM structure of the C4-symmetric TRPV2/RTx complex in amphipol resolved to 2.9 A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	TRPV2	polymer	786	88722.7	4	UniProt (G1SNM3) Pfam (PF12796) Pfam (PF00520)	Oryctolagus cuniculus (Rabbit)
2	E, F, G, H (A, B, C, D)	resiniferatoxin	non-polymer		628.7	4	Chemie (6EU) PubChem (44564218) PubChem (53776296) PubChem (57090162) PubChem (2733497) PubChem (5311411) PubChem (5702546) PubChem (176519982) PubChem (6324660) PubChem (104826) PubChem (71718531) PubChem (92845090) PubChem (5148108) PubChem (117893115) PubChem (124525003) PubChem (134688669) PubChem (15814491) PubChem (137635852) PubChem (139248250) PubChem (171342682)

Sequence modifications

A, B, C, D: 1 - 762 (UniProt: G1SNM3)

PDB	External Database	Details
Ser 470	Phe 525	engineered mutation
Ala 504	Val 559	conflict
Met 505	Leu 560	engineered mutation
Thr 508	Leu 563	engineered mutation
Glu 528	Gln 583	engineered mutation
Ser 763	-	expression tag
Asn 764	-	expression tag
Leu 765	-	expression tag
Glu 766	-	expression tag
Val 767	-	expression tag
Leu 768	-	expression tag
Phe 769	-	expression tag
Gln 770	-	expression tag
Gly 771	-	expression tag
Pro 772	-	expression tag
His 773	-	expression tag
His 774	-	expression tag
His 775	-	expression tag
His 776	-	expression tag
His 777	-	expression tag
His 778	-	expression tag
Asp 779	-	expression tag
Tyr 780	-	expression tag
Lys 781	-	expression tag
Asp 782	-	expression tag
Asp 783	-	expression tag
Asp 784	-	expression tag
Asp 785	-	expression tag
Lys 786	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	354890.7
Non-Polymers*	Number of molecules	4
	Total formula weight	2514.8
All*	Total formula weight	357405.6

*Water molecules are not included.