6OM3

Crystal structure of the Orc1 BAH domain in complex with a nucleosome core particle

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, M, Q (A, E, M, Q)	Histone H3.2	polymer	135	15303.9	4	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F, N, R (B, F, N, R)	Histone H4	polymer	103	11394.4	4	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G, O, S (C, G, O, S)	Histone H2A	polymer	130	14109.4	4	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
4	D, H, P, T (D, H, P, T)	Histone H2B 1.1	polymer	126	13979.3	4	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I, U (I, U)	DNA (146-MER)	polymer	146	44824.6	2		synthetic construct
6	J, V (J, V)	DNA (147-MER)	polymer	147	45609.1	2		synthetic construct
7	K, L, W, X (K, L, W, X)	Origin recognition complex subunit 1	polymer	224	26067.5	4	UniProt (P54784) Pfam (PF01426)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Origin recognition complex 120 kDa subunit

Sequence modifications

A, E, M, Q: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

D, H, P, T: 0 - 125 (UniProt: P02281)

PDB	External Database	Details
Thr 32	Ser 33	engineered mutation

K, L, W, X: 2 - 214 (UniProt: P54784)

PDB	External Database	Details
Ace 1	-	acetylation
Ile 79	Leu 79	engineered mutation
Ala 215	-	expression tag
Ala 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	504285.5
All*	Total formula weight	504285.5

*Water molecules are not included.