6OM3
Crystal structure of the Orc1 BAH domain in complex with a nucleosome core particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2017-10-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.254, 166.428, 168.277 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 49.054 - 3.300 |
| R-factor | 0.2167 |
| Rwork | 0.213 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tu4 1m4z 3lz1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.555 |
| Data reduction software | HKL-2000 (HKL2000_v718-Mac) |
| Data scaling software | HKL-2000 (HKL2000_v718-Mac) |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX (12-2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
| Rmerge | 0.117 | 0.081 | 0.837 |
| Rmeas | 0.142 | 0.098 | 1.014 |
| Rpim | 0.079 | 0.054 | 0.566 |
| Total number of observations | 248201 | ||
| Number of reflections | 81623 | 4163 | 4073 |
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 97.9 | 97.3 | 98.1 |
| Redundancy | 3 | 3 | 3 |
| CC(1/2) | 0.986 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277.15 | 6 mM Na-Cacodylate pH 6.0, 0.4 mM Spermine-HCl, 2 mM MgCl2 and 1.75% v/v PEG 400 |
