6OM3

Crystal structure of the Orc1 BAH domain in complex with a nucleosome core particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	110
Detector technology	PIXEL
Collection date	2017-10-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	100.254, 166.428, 168.277
Unit cell angles	90.00, 90.28, 90.00

Refinement procedure

Resolution	49.054 - 3.300
R-factor	0.2167
Rwork	0.213
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tu4 1m4z 3lz1
RMSD bond length	0.003
RMSD bond angle	0.555
Data reduction software	HKL-2000 (HKL2000_v718-Mac)
Data scaling software	HKL-2000 (HKL2000_v718-Mac)
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (12-2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.117	0.081	0.837
Rmeas	0.142	0.098	1.014
Rpim	0.079	0.054	0.566
Total number of observations	248201
Number of reflections	81623	4163	4073
<I/σ(I)>	5.2
Completeness [%]	97.9	97.3	98.1
Redundancy	3	3	3
CC(1/2)		0.986	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	6 mM Na-Cacodylate pH 6.0, 0.4 mM Spermine-HCl, 2 mM MgCl2 and 1.75% v/v PEG 400