6OK4

Crystal Structure of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Chlamydia trachomatis with bound NAD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	342	37363.7	4	UniProt (P0CE13) Pfam (PF00044) Pfam (PF02800)	Chlamydia trachomatis (strain D/UW-3/Cx)	GAPDH,NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
2	DA, E, M, U (D, A, B, C)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	BA, EA, F, FA, G... (C, D, A, B)	1,2-ETHANEDIOL	non-polymer		62.1	15	Chemie (EDO)
4	AA, GA, H, I, S... (C, D, A, B)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)
5	CA, HA, K, T (C, D, A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
6	L (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	IA, JA, KA, LA (A, B, C, D)	water	water		18.0	428	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 334 (UniProt: P0CE13)

PDB	External Database	Details
Met -7	-	expression tag
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ile 71	Val 71	engineered mutation
Arg 72	His 72	engineered mutation
Ala 105	Val 105	engineered mutation
Ile 292	Val 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	149454.7
Non-Polymers*	Number of molecules	30
	Total formula weight	4614.5
All*	Total formula weight	154069.2

*Water molecules are not included.