6OK4
Crystal Structure of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Chlamydia trachomatis with bound NAD
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006006 | biological_process | glucose metabolic process |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051287 | molecular_function | NAD binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006006 | biological_process | glucose metabolic process |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0050661 | molecular_function | NADP binding |
| B | 0051287 | molecular_function | NAD binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006006 | biological_process | glucose metabolic process |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| C | 0050661 | molecular_function | NADP binding |
| C | 0051287 | molecular_function | NAD binding |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006006 | biological_process | glucose metabolic process |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| D | 0050661 | molecular_function | NADP binding |
| D | 0051287 | molecular_function | NAD binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 27 |
| Details | binding site for residue NAD A 401 |
| Chain | Residue |
| A | GLY7 |
| A | THR96 |
| A | GLY97 |
| A | LEU98 |
| A | THR119 |
| A | ALA120 |
| A | CYS150 |
| A | ALA181 |
| A | ASN314 |
| A | TYR318 |
| A | HOH507 |
| A | PHE8 |
| A | HOH519 |
| A | HOH538 |
| A | HOH543 |
| A | HOH549 |
| A | HOH561 |
| A | HOH571 |
| A | HOH576 |
| C | HOH514 |
| A | GLY9 |
| A | ARG10 |
| A | ILE11 |
| A | ASN32 |
| A | ASP33 |
| A | LEU34 |
| A | SER95 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 402 |
| Chain | Residue |
| A | THR128 |
| A | ASN134 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 403 |
| Chain | Residue |
| A | LYS246 |
| A | HOH539 |
| B | GLU170 |
| C | ARG304 |
| C | EDO404 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | binding site for residue PO4 A 404 |
| Chain | Residue |
| A | SER149 |
| A | THR151 |
| A | THR209 |
| A | GLY210 |
| A | ALA211 |
| A | HOH509 |
| A | HOH530 |
| A | HOH537 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | binding site for residue PO4 A 405 |
| Chain | Residue |
| A | GLU170 |
| A | LYS246 |
| A | ARG304 |
| A | HOH559 |
| B | LYS246 |
| C | GLU169 |
| C | ARG304 |
| C | PO4407 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 406 |
| Chain | Residue |
| A | THR49 |
| A | ILE285 |
| C | HOH569 |
| D | THR49 |
| D | ILE285 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue PEG A 407 |
| Chain | Residue |
| A | GLY132 |
| A | VAL133 |
| A | ASN134 |
| A | HIS135 |
| A | GLN136 |
| A | GLN137 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 408 |
| Chain | Residue |
| A | HIS-1 |
| A | HIS0 |
| site_id | AC9 |
| Number of Residues | 21 |
| Details | binding site for residue NAD B 401 |
| Chain | Residue |
| B | GLY7 |
| B | GLY9 |
| B | ARG10 |
| B | ILE11 |
| B | ASN32 |
| B | ASP33 |
| B | LEU34 |
| B | SER95 |
| B | THR96 |
| B | GLY97 |
| B | LEU98 |
| B | THR119 |
| B | ALA120 |
| B | CYS150 |
| B | ALA181 |
| B | ASN314 |
| B | TYR318 |
| B | HOH534 |
| B | HOH536 |
| B | HOH553 |
| D | HOH542 |
| site_id | AD1 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 402 |
| Chain | Residue |
| B | ASN165 |
| B | ALA259 |
| B | HIS262 |
| B | ALA263 |
| B | HOH540 |
| site_id | AD2 |
| Number of Residues | 6 |
| Details | binding site for residue EDO B 403 |
| Chain | Residue |
| B | LEU163 |
| B | GLY167 |
| B | ILE168 |
| B | GLU221 |
| B | LYS225 |
| B | SER248 |
| site_id | AD3 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 404 |
| Chain | Residue |
| B | HOH504 |
| C | ASP164 |
| C | ASN165 |
| C | PHE166 |
| B | SER248 |
| B | SER249 |
| B | ALA250 |
| B | ARG304 |
| site_id | AD4 |
| Number of Residues | 2 |
| Details | binding site for residue EDO B 405 |
| Chain | Residue |
| B | TYR253 |
| B | GLY298 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 406 |
| Chain | Residue |
| A | GLN296 |
| B | ASP193 |
| B | ARG195 |
| B | HOH503 |
| B | HOH561 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 B 407 |
| Chain | Residue |
| B | SER149 |
| B | THR151 |
| B | THR209 |
| B | GLY210 |
| B | ALA211 |
| B | HOH545 |
| site_id | AD7 |
| Number of Residues | 5 |
| Details | binding site for residue PEG B 408 |
| Chain | Residue |
| B | VAL133 |
| B | ASN134 |
| B | HIS135 |
| B | GLN136 |
| B | GLN137 |
| site_id | AD8 |
| Number of Residues | 24 |
| Details | binding site for residue NAD C 401 |
| Chain | Residue |
| C | GLY7 |
| C | PHE8 |
| C | GLY9 |
| C | ARG10 |
| C | ILE11 |
| C | ASN32 |
| C | ASP33 |
| C | LEU34 |
| C | SER95 |
| C | THR96 |
| C | GLY97 |
| C | LEU98 |
| C | THR119 |
| C | ALA120 |
| C | CYS150 |
| C | ALA181 |
| C | ASN314 |
| C | TYR318 |
| C | HOH506 |
| C | HOH526 |
| C | HOH542 |
| C | HOH549 |
| C | HOH557 |
| C | HOH561 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue EDO C 402 |
| Chain | Residue |
| A | VAL35 |
| C | PRO189 |
| C | SER190 |
| C | ARG191 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue EDO C 403 |
| Chain | Residue |
| C | ASP193 |
| C | TRP194 |
| C | HOH545 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 404 |
| Chain | Residue |
| A | ARG304 |
| A | EDO403 |
| C | LYS246 |
| C | ASP303 |
| C | ARG304 |
| site_id | AE3 |
| Number of Residues | 1 |
| Details | binding site for residue EDO C 405 |
| Chain | Residue |
| C | GLN296 |
| site_id | AE4 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 C 406 |
| Chain | Residue |
| C | SER149 |
| C | THR151 |
| C | THR209 |
| C | GLY210 |
| C | ALA211 |
| C | HOH518 |
| site_id | AE5 |
| Number of Residues | 7 |
| Details | binding site for residue PO4 C 407 |
| Chain | Residue |
| A | GLU169 |
| A | ARG304 |
| A | PO4405 |
| C | GLU170 |
| C | LYS246 |
| C | ARG304 |
| D | LYS246 |
| site_id | AE6 |
| Number of Residues | 1 |
| Details | binding site for residue EDO C 408 |
| Chain | Residue |
| C | ALA300 |
| site_id | AE7 |
| Number of Residues | 6 |
| Details | binding site for residue PEG C 409 |
| Chain | Residue |
| C | GLY132 |
| C | VAL133 |
| C | ASN134 |
| C | HIS135 |
| C | GLN136 |
| C | GLN137 |
| site_id | AE8 |
| Number of Residues | 21 |
| Details | binding site for residue NAD D 401 |
| Chain | Residue |
| D | GLY7 |
| D | PHE8 |
| D | GLY9 |
| D | ARG10 |
| D | ILE11 |
| D | ASP33 |
| D | LEU34 |
| D | SER95 |
| D | THR96 |
| D | GLY97 |
| D | LEU98 |
| D | THR119 |
| D | ALA120 |
| D | CYS150 |
| D | ALA181 |
| D | ASN314 |
| D | TYR318 |
| D | HOH510 |
| D | HOH533 |
| D | HOH543 |
| D | HOH583 |
| site_id | AE9 |
| Number of Residues | 2 |
| Details | binding site for residue EDO D 402 |
| Chain | Residue |
| D | THR128 |
| D | ASN134 |
| site_id | AF1 |
| Number of Residues | 8 |
| Details | binding site for residue EDO D 403 |
| Chain | Residue |
| A | ASP164 |
| A | ASN165 |
| A | PHE166 |
| D | SER248 |
| D | SER249 |
| D | ALA250 |
| D | ARG304 |
| D | HOH560 |
| site_id | AF2 |
| Number of Residues | 7 |
| Details | binding site for residue PO4 D 404 |
| Chain | Residue |
| D | SER149 |
| D | THR151 |
| D | THR209 |
| D | GLY210 |
| D | ALA211 |
| D | HOH503 |
| D | HOH538 |
| site_id | AF3 |
| Number of Residues | 5 |
| Details | binding site for residue PEG D 405 |
| Chain | Residue |
| D | VAL133 |
| D | ASN134 |
| D | HIS135 |
| D | GLN136 |
| D | GLN137 |
Functional Information from PROSITE/UniProt
| site_id | PS00071 |
| Number of Residues | 8 |
| Details | GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL |
| Chain | Residue | Details |
| A | ALA148-LEU155 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P00362 |
| Chain | Residue | Details |
| A | CYS150 | |
| B | CYS150 | |
| C | CYS150 | |
| D | CYS150 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 36 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P00362 |
| Chain | Residue | Details |
| A | ARG10 | |
| B | ARG10 | |
| B | ASP33 | |
| B | LYS77 | |
| B | THR119 | |
| B | SER149 | |
| B | THR180 | |
| B | THR209 | |
| B | ARG232 | |
| B | ASN314 | |
| C | ARG10 | |
| A | ASP33 | |
| C | ASP33 | |
| C | LYS77 | |
| C | THR119 | |
| C | SER149 | |
| C | THR180 | |
| C | THR209 | |
| C | ARG232 | |
| C | ASN314 | |
| D | ARG10 | |
| D | ASP33 | |
| A | LYS77 | |
| D | LYS77 | |
| D | THR119 | |
| D | SER149 | |
| D | THR180 | |
| D | THR209 | |
| D | ARG232 | |
| D | ASN314 | |
| A | THR119 | |
| A | SER149 | |
| A | THR180 | |
| A | THR209 | |
| A | ARG232 | |
| A | ASN314 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Activates thiol group during catalysis => ECO:0000250|UniProtKB:P00362 |
| Chain | Residue | Details |
| A | HIS177 | |
| B | HIS177 | |
| C | HIS177 | |
| D | HIS177 |
