6OE8

The crystal structure of hyper-thermostable AgUricase mutant K12C/E286C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Uricase	polymer	326	36611.6	4	UniProt (D0VWQ1) Pfam (PF01014)	Arthrobacter globiformis	Urate oxidase,AgUOX
2	E, H, J, P, W (A, B, C, D)	TETRAETHYLENE GLYCOL	non-polymer		194.2	5	Chemie (PG4)
3	F, G, K, L, Q... (A, B, C, D)	TRIETHYLENE GLYCOL	non-polymer		150.2	8	Chemie (PGE)
4	I, M, N, O, S... (A, B, C, D)	MALONATE ION	non-polymer		102.0	8	Chemie (MLI)
5	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	1227	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 302 (UniProt: D0VWQ1)

PDB	External Database	Details
Met -23	-	initiating methionine
Ala -22	-	expression tag
Ser -21	-	expression tag
Met -20	-	expression tag
Thr -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gln -16	-	expression tag
Gln -15	-	expression tag
Met -14	-	expression tag
Gly -13	-	expression tag
Arg -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Glu -9	-	expression tag
Phe -8	-	expression tag
Met -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Cys 12	Lys 12	engineered mutation
Cys 286	Glu 286	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146446.6
Non-Polymers*	Number of molecules	21
	Total formula weight	2988.9
All*	Total formula weight	149435.5

*Water molecules are not included.