6OE8

The crystal structure of hyper-thermostable AgUricase mutant K12C/E286C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004846	molecular_function	urate oxidase activity
A	0006144	biological_process	purine nucleobase metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0019628	biological_process	urate catabolic process
B	0004846	molecular_function	urate oxidase activity
B	0006144	biological_process	purine nucleobase metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0019628	biological_process	urate catabolic process
C	0004846	molecular_function	urate oxidase activity
C	0006144	biological_process	purine nucleobase metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0019628	biological_process	urate catabolic process
D	0004846	molecular_function	urate oxidase activity
D	0006144	biological_process	purine nucleobase metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0019628	biological_process	urate catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue PG4 A 401

Chain	Residue
A	LYS250
A	ALA272
A	ASP273
A	TYR276

---

site_id	AC2
Number of Residues	8
Details	binding site for residue PGE A 402

Chain	Residue
B	ASP256
B	HOH663
D	PRO275
A	LYS71
A	ASN72
A	TYR75
A	HOH556
A	HOH693

---

site_id	AC3
Number of Residues	10
Details	binding site for residue PGE A 403

Chain	Residue
A	GLU24
A	ASN43
A	THR45
A	ALA109
A	ALA110
A	THR134
A	ARG189
A	HOH508
A	HOH530
A	HOH646

---

site_id	AC4
Number of Residues	6
Details	binding site for residue PG4 A 404

Chain	Residue
A	LEU254
A	ASN264
A	HOH535
C	GLN262
C	ASP263
D	ARG79

---

site_id	AC5
Number of Residues	9
Details	binding site for residue MLI A 405

Chain	Residue
A	ALA66
A	THR67
A	ASP68
A	HOH518
A	HOH686
D	PHE163
D	LEU174
D	ARG180
D	ASN249

---

site_id	AC6
Number of Residues	3
Details	binding site for residue PG4 B 401

Chain	Residue
B	LYS250
B	ASP273
B	TYR276

---

site_id	AC7
Number of Residues	5
Details	binding site for residue PGE B 402

Chain	Residue
A	VAL255
B	LYS71
B	ASN72
B	HOH557
C	PHE253

---

site_id	AC8
Number of Residues	9
Details	binding site for residue PGE B 403

Chain	Residue
B	ASN43
B	THR45
B	ALA109
B	ALA110
B	GLN111
B	THR134
B	ARG189
B	HOH549
B	HOH607

---

site_id	AC9
Number of Residues	10
Details	binding site for residue MLI B 404

Chain	Residue
B	ALA66
B	THR67
B	ASP68
B	HOH601
B	HOH623
C	PHE163
C	LEU174
C	ARG180
C	ASN249
C	HIS251

---

site_id	AD1
Number of Residues	10
Details	binding site for residue MLI C 401

Chain	Residue
B	ARG274
C	ARG26
C	PHE113
C	ARG127
C	HOH512
C	HOH581
C	HOH614
C	HOH694
C	HOH695
D	PG4403

---

site_id	AD2
Number of Residues	9
Details	binding site for residue MLI C 402

Chain	Residue
B	PHE163
B	LEU174
B	ARG180
B	ASN249
B	HIS251
C	ALA66
C	THR67
C	ASP68
C	HOH524

---

site_id	AD3
Number of Residues	8
Details	binding site for residue PG4 C 403

Chain	Residue
A	ARG274
B	HOH519
C	LYS250
C	ALA272
C	ASP273
C	TYR276
C	HOH522
C	HOH673

---

site_id	AD4
Number of Residues	9
Details	binding site for residue PGE C 404

Chain	Residue
D	TYR270
B	HIS251
B	PHE253
B	PRO275
C	LYS22
C	LYS71
C	TYR75
C	HOH514
C	HOH538

---

site_id	AD5
Number of Residues	11
Details	binding site for residue PGE C 405

Chain	Residue
C	GLU24
C	ASN43
C	THR45
C	ALA109
C	ALA110
C	GLN111
C	ARG189
C	HOH501
C	HOH533
C	HOH543
C	HOH653

---

site_id	AD6
Number of Residues	7
Details	binding site for residue MLI C 406

Chain	Residue
B	ARG26
B	PHE113
B	ARG127
B	HOH530
B	HOH558
B	HOH577
C	ARG274

---

site_id	AD7
Number of Residues	4
Details	binding site for residue PGE C 407

Chain	Residue
C	VAL255
C	ASP256
D	LYS71
D	ASN72

---

site_id	AD8
Number of Residues	7
Details	binding site for residue MLI D 401

Chain	Residue
A	ARG274
D	ARG26
D	PHE113
D	ARG127
D	HOH568
D	HOH572
D	HOH688

---

site_id	AD9
Number of Residues	12
Details	binding site for residue MLI D 402

Chain	Residue
A	PHE163
A	LEU174
A	ARG180
A	ASN249
A	HIS251
A	HOH670
D	ALA66
D	THR67
D	ASP68
D	HOH603
D	HOH703
D	HOH708

---

site_id	AE1
Number of Residues	7
Details	binding site for residue PG4 D 403

Chain	Residue
B	ARG274
C	MLI401
D	LYS250
D	ALA272
D	ASP273
D	TYR276
D	HOH508

---

site_id	AE2
Number of Residues	10
Details	binding site for residue PGE D 404

Chain	Residue
D	GLU24
D	ASN43
D	THR45
D	ALA109
D	ALA110
D	GLN111
D	ARG189
D	HOH506
D	HOH549
D	HOH609

---

site_id	AE3
Number of Residues	9
Details	binding site for residue MLI D 405

Chain	Residue
A	ARG26
A	PHE113
A	ARG127
A	HOH536
A	HOH613
A	HOH688
D	ARG274
D	HOH556
D	HOH630

---

Functional Information from PROSITE/UniProt

site_id	PS00366
Number of Residues	28
Details	URICASE Uricase signature. LtVLKSTgSeFhgFprdkYttLqettdR

Chain	Residue	Details
A	LEU153-ARG180

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"18645230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18645230","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2YZB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2YZC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q00511","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---